Protocols for molecular biology
General rules and manners at Yamamoto's Lab.
Media
LB, YT medium
Syncase medium
M9 medium
Cultivation methods of bacteria
Molecular Genetics
Treatment of DNA
Ethenol precipitation of DNA
Deproteinization of DNA preparation with phenol and chlorform treatment
Elution of DNA from PAGE
Extraction of DNA from agarose gel with beta-agarase I
Electrophoresis of DNA
Agarose
PAGE
Buffers for electrophresis
Preparation of genomic and plasmid DNA
Plasmid DNA (Mini-prep)
50 ml-scale
Plasmid DNA (Medium-scale)
Plasmid DNA (CsCl centrifugation)
Plasmid DNA (Large-scale, conventional)
Bacterial genomic DNA (small-scale)
Bacterial genomic DNA (Large-scale)
Enzymatic reactions
Bacterial alkaline phosphatase
Transformation
Transformation of
E. coli
Use of frozen
E. coli
competent cells
Transformation of
E. coli
strains (for >5 sample, classical method)
Simplified transformation of
E. coli
strains (for 1 sample)
Ten-minutes transformation using frozen competent cells
Preparation of frozen storages of
E. coli
competent cells(Rb-Ca method)
Electroporation
Electroporation of
E. coli
by electroporation
Preparation of storage of competent cell for electroporation
Transfomation of vibrios by electrotion
Hybridization
Southern blot transfer
Direct labeling of probes with alkaline phosphatase
Transfer of colony to nylon membrane
Hybridization with Dig-labeled probes
Protein Chemistry
SDS-PAGE
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